RNA Isolation Kit
| Product | Cell-Mate3D RNA Isolation Standard Kit With Ceramic Beads | Cell-Mate3D RNA Isolation Standard Kit Without Ceramic Beads |
|---|---|---|
| Product Number | RXT-1000 | RXT-1001 |
| Unit Size | 30 Reactions | 30 Reactions |
| List Price | $150.00 | $99.00 |
| Add to Cart | Add to Cart |
Optimized RNA extraction from Cell-Mate3D™ cultures
Reagents and workflow are optimized for efficient RNA isolation and recovery from Cell-Mate3D™ cultures. Protocol is derived from methods extracting RNA from polysaccharide rich tissues. 1,2,3
Efficient RNA isolation & recovery
Cell-Mate3D™ has unique chemical and physical properties such that common RNA isolation kits are not suitable for RNA extraction.
Isolate RNA with Minimal Protein Contamination
Nanodrop spectra of isolated RNA from 7 individual Cell-Mate3D™ samples with Varying cell densities. The total RNA yield represented range from 1.8ug to 16.8ug.
Obtain High Quality RNA after Qiagen® RNA Cleanop
Agilent Bioanalyzer electropherogram depicting high quality total RNA isolated from Cell-Mate3D™ culture followed by Qiagen® RNA clean-up. Note clearly delineated 28S and 18S peaks and minimal low molecular weight noise. rRNA Ratio 28S/18S=2.2
Synthesize cDNA and Perform q-RT PCR
q-RT-PCR amplification plot demonstrating GAPDH expression. RNA was isolated from Cell-Mate3D™ Cultures and converted to cDNA. 200ng of cDNA per well was used in each q-RT-PCR reaction. Technical triplicates were performed. Average CT value was 15.5.
ProtocolContents:
- Reagent A: Dextran Sulfate, 2 ml vial
- Reagent B: Extraction Buffer, 10 ml brown vial
- CHCl3: Isoamyl Alcohol (24:1), 60 ml reagent bottle
- Reagent C: 7M LiCl, 2 ml vial
- Reagent D: 4M GuHCl, 10 ml brown vial
- Reagent E: 0.4M LiCl, 10 ml brown vial
- Glassmilk Suspension: 440 mg/ml, 2 ml vial
- Precellys Ceramic Bead Tubes
