3D Cell Culture in a Self-Assembled Nanofiber Environment.


Authors:  Yi Wen Chai, Eu Han Lee, John D. Gubbe, and John H Brekke

Journal:  PLOS ONE

Date:  9/15/2016

Learn how Cell-Mate3D™ can be used to create tumor models. Complex 3D cultures are analyzed using flow cytometry, immunostaining, histological staining, and migration studies. The article also demonstrates how the Young’s Modulus of the construct is measured.


Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium.


Authors:  Beth A. Lindborg, John H. Brekke, Amanda L. Vegoe et al.

Journal:  Stem Cells Translational Medicine

Date:  6/13/2016

Cell-Mate3D™ can be used to generate cerebral organoids using only E8 culture medium.  Organoids were analyzed for gene expression using RT-PCR, IHC, and Fluorescent Microscopy.

Poster Publications

Application of Cell-Mate™ 3D matrix to modeling artificial breast cancer and “normal” ductal tissues.


Authors:  Timothy Lyden, Bruna Stilpen Justen, Ronaldo Loureiro
Meeting:  Experimental Biology 2016

Breast cancer and normal ductal tissue are modeled using MCF-7 and MCF10A cell lines in conjunction with the Cell-Mate3D™ matrix. In the case of MCF-7 cells, a tumor architecture was observed. In the case of MCF10A cells, a ductal like architecture was observed. The study demonstrates an effective approach to modeling breast cancer tumors and tumor microenvironments.


IGF-1 and TGF-? promote EMT and angiogenesis in 3D cultures of lung generic clomid capsules online clomid online pharmacy adenocarcinoma cells: A pilot study.


Authors:  Imad Tarhoni, Gabriela Lobato, Cristina Fhied, et al.
Meeting:  AACR Annual Meeting 2016

Lung cancer tumors are modeled in this study using A549 cells embedded into Cell-Mate3D™. Cultures were treated with Transforming Growth Factor beta (TGF-?) and Insulin Like Growth Factor 1 (IGF-1), which are well-established inducers of EMT and promoters of angiogenesis.  Effects of these growth factors are analyzed using Milliplex® technology.


Culture and Trilineage Differentitation of hMSCs Within a Novel 3D Microenvironment. Applications for in vitro and in vivo Biomedical Research.


Authors: Beth A. Lindborg, Yi Wen Chai, Connor B. Ulrich

Meeting: Hilton Head Regenerative Medicine Conference 2015

Benefits of 3D cell culture and the use of Cell-Mate3D™ is highlighted in this study. Trilineage differentiation of hMSCs in Cell-Mate3D™ and downstream applications such as RT-PCR, fluorescent microscopy, and injection/biocompatibility are demonstrated.


Hyaluronan-Chitosan Polyelectrolytic Complex as a Platform for Delivery of Cytotoxic Agents


Authors: Sarah Philen, Rebecca Coleman, Aderinsola Gilbert, Katy McFadden, Shaili Sharma, Eric Stensgard, John Brekke, Gregory Rutkowski.

Meeting: Gordon Conference 2007

This in vitro study demonstrates how Cell-Mate3D™ can be used as a drug delivery platform. The study shows how Paclitaxel incorporated into the Cell-Mate3D™ matrix is cytotoxic to malignant melanoma cells.


Creating a Dynamic Tumor Microenvironment with Cell-Mate3D.


Guest Speaker:  Dr. Ehab Sarsour, University of Iowa

This webinar demonstrates how Cell-Mate3D™ can be used to create tumor microenvironments and predictive models for therapeutic discoveries.

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