Cell-Mate3D Gel 40™ Kit

Cell-Mate3D µGel 40™Kit

Cell-Mate3D µGel 40™ Kit

Product Cell-Mate3D µGel 40 Pack of 8 Cell-Mate3D µGel 40 Pack of 24
Product Number CM-401 CM-402
Unit Size Creates 8 microgels, each ~40μL (total vol./pack 0.32mL) Creates 24 microgels, each ~40μL (total vol./pack 0.96mL)
List Price $49.00 $132.00
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Cell-Mate3D µGel 40™ provides your cells with an ideal microenvironment for disease modeling and regenerative medicine research. It is ideal for cancer cells to model the tumor microenvironment and for stem cells to differentiate toward Central Nervous System tissue. The unique microgel design takes advantage of its chemically defined, tissue-like, biomimetic 3D technology. Thus, allowing for use as a quantitative tool with fewer cells (lower limit 12K) embedded within the 3D matrix. Moreover, the simplicity and ease of use offers a fast set-up in as few as 5 minutes unlike other 3D products in the market.

ADVANCE YOUR RESEARCH WITH CELL-MATE3D µGEL 40™

  • Simple and easy setup at room temperature
  • Tissue-like stiffness better mimics in vivo extracellular environment
  • Allows for emergence of complex microtissues/organoids
  • Chemically Defined and Quantifiable Tool
  • Replace up to 20%* of hydration fluid with other ECM components for further customization

*May affect matrix stiffness

Cell-Mate3D µGel 40™Kit

Advantages over other 3D culture options

  • Cost effective alternative to other 3D scaffolds or gels
  • No chemical or UV crosslinking agents
  • Use both in vitro and in vivo
  • Biocompatible
  • Permits long-term 3D cultures without producing a necrotic core (depending on cell type)
  • Works with low cell volumes (populations) lower limit 12K cells/upper limit 1M cells
  • Cell-Mate3D technology offers translational capability because individual components have Drug Master Files with the FDA

Cell types that have been cultured in Cell-Mate3D

Primary Cancer Cells

  • Fibrosarcoma (HT1080)
  • Melanoma (C8161)
  • Glioblastoma (GBM-22)
  • B-Lymphoma
  • Rare Brain Tumor
  • Colon Cancer
  • Breast Cancer
  • Pancreatic Cancer
  • Ovarian cancer

Stem Cells

  • Human iPSCs
  • Human MSCs
  • Mouse ESCs
  • Mouse MSC
  • Canine MSC
  • Exocrine primary progenitor cells
  • 3T3 Mouse Fibroblasts
  • Alveolar cells (pneumocytes type I/II)
  • Retinal rods (human primary)

Cancer Cell Lines

  • Breast cancer (MCF7)
  • HeLa
  • Kidney (HEK293)
  • Pancreatic Cancer (MiaPaca-2)
  • Lung adenocarcinoma (A549)
  • Lung adenocarcinoma (HCC358)
  • Bladder (T24)
  • AU565

CELL EMBEDDING PROCESS – WORK FLOW DIAGRAM

Cell Embedding Process

Step 1.  Prepare plate – remove cap and separate 1 microwell from strip via break-away vial

Step 2.  Prepare cells – mix cell pellet with hydration fluid using pipette

Step 3.  Embed cells – center the pipet tip over the channel opening and add cells in hydration fluid to the µGel 40 construct

Step 4.  Transfer gel – after 5 minutes cure time use sterile tweezers gently pick up gel and transfer to well plate of choice and add media of choice

Analyze your Cell-Mate3D™ cultures using common laboratory techniques.

Contents:
Each Cell-Mate3D kit Contains:

  • 8 or 24 vials of pre-formatted µGel 40
  • Product delivered in strips of 8 vials (i.e. 1×8 or 3×8 strips)
  • 1 Tube of Hydration Fluid

Specifications:

  • Microbial Purity / Sterile / USP 71/85
  • pH / 5.0-7.0 / ASTM DS 148
  • Storage Conditions / 4°C / Recommended
  • Shelf Life / 2 years / ASTM F2347, ASTM F2103