For Phone Orders Call 1-888-868-9100 or Contact Us

Cancer Stem Cell Biology Applications

Cell-Mate3D™ Cancer Stem Cell Biology Applications

Create more predictive models and uncover complex, biologically relevant mechanisms with Cell-Mate3D™. Analyze your Cell-Mate3D™ cultures using common laboratory tools and techniques. Visualize with microscopy, and analyze gene expression and cell dynamics using our optimized and easy-to-follow protocols.

RNA Isolation Kit

Isolate RNA utilizing our proprietary Cell-Mate3D RNA Isolation Kit™. Because Cell-Mate3D™ is highly charged, reagents and work-flow have been optimized for efficient isolation and recovery. Additional cleanup may be required (ie, Qiagen™ Cleanup Kit Cat #74204) depending on downstream application. Analyze RNA expression using RT-PCR, qRT-PCR, RNA Seq, and other RNA based assays.

Protein Isolation

Extract protein directly from Cell-Mate3D™ using our optimized protocol or analyze secreted proteins in cell culture media. Analyze protein expression using Western Blotting and other assays

Matrix Immobilization: Plate Bottom Protocol

Does your application require that your 3D culture be fixed to the bottom of a plate? This easy to follow protocol will immobilize your 3D culture. Ideal for assays where interaction between cells inside the matrix and cells added to media outside of the matrix is desired.

Microscopy and Staining

Visualize your discoveries using light microscopy*, confocal microscopy, and electron microscopy. As Cell-Mate3D™ is tissue-like, samples can be paraffin embedded, cryosectioned, and stained using standard reagents and our optimized protocols.
*Requires extra step during set up. See Light Microscopy protocol.

Whole Matrix Staining & Confocal Microscopy Protocol

Quantification of Live/Dead Staining using Fiji Software

Cryosectioning & Staining Protocol

Electron Microscopy Protocol

Live/Dead Staining Protocol

BRTI Life Sciences - Logo

Sign up for the BRTI Newsletter to get actionable advice
and exclusive deals direct to your inbox!