Cancer Stem Cell Biology Applications

Cell-Mate3D™ Cancer Stem Cell Biology Applications

Create more predictive models and uncover complex, biologically relevant mechanisms with Cell-Mate3D™. Analyze your Cell-Mate3D™ cultures using common laboratory tools and techniques. Visualize with microscopy, and analyze gene expression and cell dynamics using our optimized and easy-to-follow protocols.

RNA Isolation Kit

 

Isolate RNA utilizing our proprietary Cell-Mate3D RNA Isolation Kit™. Because Cell-Mate3D™ is highly charged, reagents and work-flow have been optimized for efficient isolation and recovery. Additional cleanup may be required (ie, Qiagen™ Cleanup Kit Cat #74204) depending on downstream application. Analyze RNA expression using RT-PCR, qRT-PCR, RNA Seq, and other RNA based assays.

3D Cell Culture Protocol for Analyzing RNA Expression

Protein Isolation

 

Extract protein directly from Cell-Mate3D™ using our optimized protocol or analyze secreted proteins in cell culture media. Analyze protein expression using Western Blotting and other assays

3D Cell Culture Protocol for Analyzing Protein Expression

Matrix Immobilization: Plate Bottom Protocol

Does your application require that your 3D culture be fixed to the bottom of a plate? This easy to follow protocol will immobilize your 3D culture. Ideal for assays where interaction between cells inside the matrix and cells added to media outside of the matrix is desired.

3D Cell Culture Protocol for Matrix Immobilization

Microscopy and Staining

 

Visualize your discoveries using light microscopy*, confocal microscopy, and electron microscopy. As Cell-Mate3D™ is tissue-like, samples can be paraffin embedded, cryosectioned, and stained using standard reagents and our optimized protocols.

*Requires extra step during set up. See Light Microscopy protocol.

3D Cell Culture Protocol Using Microscopy and Staining
Whole Matrix Staining & Confocal Microscopy Protocol
Quantification of Live/Dead Staining using Fiji Software
Cryosectioning & Staining Protocol
Electron Microscopy Protocol
Live/Dead Staining Protocol