For Phone Orders Call 1-888-868-9100 or Contact Us

RNA Isolation Kit

RNA Isolation Kit

$99.00$150.00

Trusted By

 

 

SKU: N/A Category:

Description

Overview

Optimized RNA extraction from Cell-Mate3D™ cultures

Reagents and workflow are optimized for efficient RNA isolation and recovery from Cell-Mate3D™ cultures. Protocol is derived from methods extracting RNA from polysaccharide rich tissues. 1,2,3

Efficient RNA isolation & recovery

Cell-Mate3D™ has unique chemical and physical properties such that common RNA isolation kits are not suitable for RNA extraction.

Unite Size

Standard Kit With Ceramic Beads: 30 Reactions
Standard Kit Without Ceramic Beads: 30 Reactions

Isolate RNA with Minimal Protein Contamination

Nanodrop spectra of isolated RNA from 7 individual Cell-Mate3D™ samples with Varying cell densities. The total RNA yield represented range from 1.8ug to 16.8ug.

Obtain High Quality RNA after Qiagen® RNA Cleanop

Agilent Bioanalyzer electropherogram depicting high quality total RNA isolated from Cell-Mate3D™ culture followed by Qiagen® RNA clean-up. Note clearly delineated 28S and 18S peaks and minimal low molecular weight noise. rRNA Ratio 28S/18S=2.2

Synthesize cDNA and Perform q-RT PCR

q-RT-PCR amplification plot demonstrating GAPDH expression. RNA was isolated from Cell-Mate3D™ Cultures and converted to cDNA. 200ng of cDNA per well was used in each q-RT-PCR reaction. Technical triplicates were performed. Average CT value was 15.5.

Contents and Specifications

  • Reagent A: Dextran Sulfate, 2 ml vial
  • Reagent B: Extraction Buffer, 10 ml brown vial
  • CHCl3: Isoamyl Alcohol (24:1), 60 ml reagent bottle
  • Reagent C: 7M LiCl, 2 ml vial
  • Reagent D: 4M GuHCl, 10 ml brown vial
  • Reagent E: 0.4M LiCl, 10 ml brown vial
  • Glassmilk Suspension: 440 mg/ml, 2 ml vial
  • Precellys Ceramic Bead Tubes

Frequently Bought Together

Reviews

High Quality Product

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry’s standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged.

Have questions about our

RNA Isolation Kit?

A BRTI Expert is happy to help!

Questions? Call 1-888-868-9100 or Contact Us

BRTI Life Sciences - Logo

Sign up for the BRTI Newsletter to get actionable advice
and exclusive deals direct to your inbox!

Additional information

Select Size

Cell-Mate3D RNA Isolation Standard Kit With Ceramic Beads, Cell-Mate3D RNA Isolation Standard Kit Without Ceramic Beads