Description
Overview
Optimized RNA extraction from Cell-Mate3D™ cultures
Reagents and workflow are optimized for efficient RNA isolation and recovery from Cell-Mate3D™ cultures. Protocol is derived from methods extracting RNA from polysaccharide rich tissues. 1,2,3
Efficient RNA isolation & recovery
Cell-Mate3D™ has unique chemical and physical properties such that common RNA isolation kits are not suitable for RNA extraction.
Unite Size
Standard Kit With Ceramic Beads: 30 Reactions
Standard Kit Without Ceramic Beads: 30 Reactions
Isolate RNA with Minimal Protein Contamination
Nanodrop spectra of isolated RNA from 7 individual Cell-Mate3D™ samples with Varying cell densities. The total RNA yield represented range from 1.8ug to 16.8ug.
Obtain High Quality RNA after Qiagen® RNA Cleanop
Agilent Bioanalyzer electropherogram depicting high quality total RNA isolated from Cell-Mate3D™ culture followed by Qiagen® RNA clean-up. Note clearly delineated 28S and 18S peaks and minimal low molecular weight noise. rRNA Ratio 28S/18S=2.2
Synthesize cDNA and Perform q-RT PCR
q-RT-PCR amplification plot demonstrating GAPDH expression. RNA was isolated from Cell-Mate3D™ Cultures and converted to cDNA. 200ng of cDNA per well was used in each q-RT-PCR reaction. Technical triplicates were performed. Average CT value was 15.5.
Protocol/SDS
Contents and Specifications
- Reagent A: Dextran Sulfate, 2 ml vial
- Reagent B: Extraction Buffer, 10 ml brown vial
- CHCl3: Isoamyl Alcohol (24:1), 60 ml reagent bottle
- Reagent C: 7M LiCl, 2 ml vial
- Reagent D: 4M GuHCl, 10 ml brown vial
- Reagent E: 0.4M LiCl, 10 ml brown vial
- Glassmilk Suspension: 440 mg/ml, 2 ml vial
- Precellys Ceramic Bead Tubes
Frequently Bought Together
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Cell-Mate3D™ Kits
$195.00 – $285.00 -
Cell-Mate3D µGel 40™ Kit
$49.00 – $132.00 -
Cell Retrieval Kit
$48.00 -
RNA Isolation Kit
$99.00 – $150.00
Reviews
High Quality Product
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RNA Isolation Kit?
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