Scientists have mastered the art of chemical signaling in 2D culture to recapitulate disease and development. However, it has become increasingly apparent that the emergence of more complex structures and micro-tissues requires mechanical signaling from a tissue-like, 3D environment[1],[2].
Recent examples include neuronal organoid development[3], optic cup morphogenesis[4], gut organoid development[6], and liver buds[7]. These “emergent micro-tissues” hold promise for both therapeutic applications as well as in vitro disease models.
Cell-cell and cell-matrix adhesion[8]
Mechanical signaling[9]
Chemical and gas diffusion gradients[10]
Germ layer regulation and signaling[11]
Complex patterning[12]
Culture cells in Cell-Mate3DTM and propagate in media of choice. Cell-Mate3DTM is ideal for:
Cell-Mate3DTM Has Advantages Over Other 3D Options Because It:
Researchers benefit because Cell-Mate3DTM is:
[1]Brendon M. Baker et al. Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues. J Cell Sci 2012. 125(Pt 13):3015-3024.
[2] Marius Ader et al. Modeling human development in 3D culture. Curr Opin Cell Biol 2014. 31:23–28
[3]Lancaster MA et al.Cerebral organoids model human brain development and microcephaly. Nature 2013. 501(7467):373-379
[4] Mototsugu Eiraku et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature 2011. 472(7341):51-56
[5] Tokushige Nakano et al. Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs. Cell Stem Cell 2012. 10(6):771-785
[6] Spence et al.: Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature 2011. 470(7332):105-109.
[7] Takanori Takebe et al. Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant. Nat Protoc 2014. (2):396-409
[8] Oliver Zschenker et al. Genome-Wide Gene Expression Analysis in Cancer Cells Reveals 3D Growth to Affect ECM and Processes Associated with Cell Adhesion but Not DNA Repair. PLoS One 2012. 7(4):e34279
[9] Brendon M. Baker. Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues. J Cell Sci. 2012. 125(Pt 13):3015-24.
[10] Brendon M. Baker et al. Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues. J Cell Sci 2012. 125(Pt 13):3015-3024.
[11] Yeh-Chuin Poh. Generation of organized germ layers from a single mouse embryonic stem cell. Nat Commun. 2014. 5:4000.
[12] Koehler, K.R., et al., Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture. Nature. 2013. 500(7461):217-221
